The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

GeneFisher—fishing for genes, perhaps? Fishing genes out of genomes? Just fishing? The subtitle, Interactive PCR Primer Design, “hooked me” (get it?) but it shouldn’t have in retrospect. After all, ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

The polymerase chain reaction -- A brief comparison between in vivo DNA replication and in vitro PCR amplification -- The PCR in practice -- The different types and varieties of nucleic acid target ...

This webinar provides a practical introduction to the fundamentals of assay design for quantitative real-time PCR (qPCR), with a strong focus on optimizing specificity and performance. Attendees will ...